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Galaxy is an open, web-based platform designed for FAIR data analysis, in biomedical research originally but spanning numerous scientific domains nowadays.

There is worldwide network of Galaxy servers providing open access to virtually all academic users consisting of "copies" (instances) of the service in United States, Europe and Australia. Besides, numerous specialized services exist.

Extensive documentation, including quickstart and advanced tutorials, is available on Galaxy Community Hub.

E-infraCZ / Metacentrum together with Elixir CZ provides the service. It aims at replicating the functionality (set of available tools in particular) of the worldwide services (, while offering significantly higher user quotas (both computational and storage) to the registered CZ users and their collaborators.

Federated Login Options

Metacentrum Galaxy provides two convenient options for logging in:

  1. E-infra AAI which is the prefered way for CZ academic users, and it grants higher computing and storage quotas automatically.
  2. Life Science Login If you are associated with the LifeScience/Elixir, you can log in using LifeScience AAI. This is the same method used by and it grants access to the same set of users, with restricted quotas, though.

CZ users, who are able to use E-infra AAI, are able to login also with Life Science Login in most cases, and they are advised to link them together in Galaxy to avoid future confusion (e.g. not being able to access results stored previously).

The following procedure links the identities:

  • Go to your Galaxy user settings by clicking on Preferences in the User tab.
  • Under Preferences, select Manage Third-Party Identities.
  • Select the other identity provider and authenticate yourself.

FTP Access

Standard file upload to Galaxy via web browser (through the Upload data button) can be rather limiting for huge files or large number of files. These cases must be addressed with FTP upload instead. The process is documented here, and unlike it has not been decomissioned at

On the other hand, FTP support in Galaxy is rather legacy, and it does not provide smooth integration with the federated login. Instead, several setup steps are required to use it at the first time:

  1. Initial Setup:

    • When you first register on Metacentrum Galaxy, you won't have an FTP password by default.
  2. Reset Your Password:

    • To set up your FTP access, go to the Galaxy login panel.
    • Enter your registered email address into the login panel.
  3. Reset Your Password via Email:

    • Click on the "Click here to reset your password" button.
  4. Check Your Email:

    • You will receive an email with a reset password link. Please check your email inbox, including your spam folder, if necessary.
  5. Set a New Password:

    • Open the email and click on the provided link.
    • You will be directed to a page where you can set up a new password for your FTP access.
  6. FTP Access:

    • Once you have set a new password, you can use your registered email address and the new password to log in via FTP.

Now, you can use your email address and password to upload files to Metacentrum Galaxy using your preferred FTP client.

Resource availability and user quotas


Data storage reliability

In a nutshell, data storage of is resilient to "normal" disk failures, common consistency problems following abrupt power outages etc. However, the all the data are still stored in a single server room, and they are neither replicated nor backed up elsewehere. Therefore, abnormal situation (fire in the server room etc.) may lead to data loss. Hence, users are not advised to use this service to store high-value data in a single copy, and to rely on the storage for long term.

In more technical detail, both the user data files and the Galaxy database are stored on remote block device (RBD) provided by CESNET storage department. Specifically, RBD is configured as 12+4 Ceph erasure code, hence it is able to recover from simultaneous failure of four drives. Further, regular RBD snapshots are created to mitigate the risk of ending up with irrecoverable filesystem corruption.



Old documentation

Metacentrum used to operate a legacy Galaxy instance till 2023. Its documentation is preserved here for eventual reference.


If you need any help or experience tool errors or any unexpected issues contact us at